Highly selective and sensitive adenosine aptasensor based on platinum nanoparticles as catalytical label for amplified detection of biorecognition events through H₂O₂ reduction

چکیده مقاله

Based on a combination of aptamer and platinum nanoparticles a new sensitive and selective adenosine aptasensor was fabricated. Functionalized thiol-terminated adenosine aptamer (5'-AGAGAACCTGGGGGAGTATTGCGGAGGAAGGT-SH-3') with Pt Nanoparticles (Pt-NPs) was employed as highly catalytic label for electrochemical detection of adenosine based on electrocatalytic activity of Pt-NPs toward H₂O₂ reduction. Multiwalled carbon nanotubes/ionic liquid/chitosan (MWCNTs/IL/CHIT) nanocomposite was applied as the interface for covalent attachment of 3'-amine-terminated capture probe (3'-NH₂-(CH₂)₆-TCTCTTGGACCC-5'). The presence of Pt nanoparticles improvement the conductivity and performance characteristics of the biosensor as well as incensement in the loading amount of the aptamer DNA sequence. The interaction of adenosine with the aptamer causes the releasing of aptamer with PtNPs into solution which resulted in a decreasing of hydrogen peroxide reduction peak current. Sensitive quantitative detection of adenosine was achieved by monitoring the decrease of voltammetric responses of H₂O₂ peak current. The peak current of H₂O₂ decreased with increase in the concentration of adenosine over a range of 1-750nM with detection limit 1nM. In addition the proposed aptasensor showed excellent selectivity toward adenosine in compared to some other nucleosides such as guanosine, cytidine and uridine. The proposed aptasensor was successfully used to detect adenosine in human serum samples. The elimination of enzymes or antibodies for the amplified detection of adenosine and the use of platinum nanoparticles as inorganic catalytic label, are the advantage of the proposed aptasensor. © 2013 Elsevier B.V.