Investigating the effect of trans-anethole on the expression changes of TNF-α, resistin, and chemerin genes in visceral adipose tissue caused by estradiol valerate injection in female wistar rats

چکیده مقاله

Background. Polycystic ovary syndrome (PCOS) is one of the endocrine diseases of the reproductive system, with a prevalence of 5‒10% in women, and one of its symptoms is an increase in the serum amount of pro-inflammatory adipokines from adipose tissue. Methods. This study was performed on six groups of 6 female rats, including a sham group, an estradiol valerate experimental group that received a concentration of 2 mg per kg of estradiol valerate, and two trans-anethole experimental groups that received concentrations of 50 mg/kg and 80 mg/kg of trans-anethole. The other groups were two experimental groups of estradiol valerate treated with trans-anethole that received concentrations of 50 mg/kg and 80 mg/kg of trans-anethole 60 days after receiving estradiol valerate. The data were statistically analyzed with a one-way analysis of variance and Tukey’s post hoc test via SPSS. Results. Increased expression of tumor necrosis factor-alpha (TNF-α), chemerin, and resistin genes in the visceral fat tissue of rats caused by the intramuscular injection of estradiol valerate was reduced by the intraperitoneal injection of concentrations of 50 mg/kg and 80 mg/kg of trans-anethole (P < 0.05). Conclusion. Trans-anethole ameliorated the expression of TNF-α, chemerin, and resistin genes in the visceral fat tissue of female rats suffering from PCOS. Practical Implications. The use of trans-anethole as an extract of anise and fennel may be effective in reducing the inflammatory complications of women with PCOS. © 2025 The Authors.